Gruft Mycobacterial Supplement
Rehydrate the contents of 1 vial aseptically with 2.5 ml sterile distilled water. Appropriate concentrations of the
supplement can be achieved by absorbing 0.05 ml of reconstituted supplement on slants prepared from 8 ml L-J Medium.
(slants prepared with 150 ml of sterile TM 375 - LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) / TMV 375 -
LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) (VEG.) / TM 526 - L.J. MEDIUM W/O. STARCH (as per Intertiol
Standard) / TM 2167 - LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) (as per IP) (DOUBLE PACK) and 250 ml of
whole egg emulsion collected aseptically).
Rehydrate the contents of 1 vial aseptically with 2.5 ml sterile distilled water. Appropriate concentrations of the
supplement can be achieved by absorbing 0.05 ml of reconstituted supplement on slants prepared from 8 ml L-J Medium.
(slants prepared with 150 ml of sterile TM 375 - LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) / TMV 375 -
LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) (VEG.) / TM 526 - L.J. MEDIUM W/O. STARCH (as per Intertiol
Standard) / TM 2167 - LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) (as per IP) (DOUBLE PACK) and 250 ml of
whole egg emulsion collected aseptically).
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