Gc Agar Base

-Dissolve the 36.0 grams in 500 ml purified / distilled water, to make a double strength base. -Heat to boiling to dissolve the medium completely. -Separately suspend 10 grams of Haemoglobin powder in 500 ml purified / distilled water (2% solution) to make a uniform solution. Separately sterilize both the solutions by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C. -Mix both the solutions uniformly and aseptically add the rehydrated contents of two vials of GC Supplement w/ Antibiotics. Mix well and pour into sterile Petri plates. -To increase the selectivity of medium antibiotic supplements such as V.C.N. Supplement or V.C.N.T. Supplement or Linco T Supplement or Vanclo T Supplement (two vials per litre) may be added. -To enhance the nutritiol properties of medium, Vitamins amino growth supplement or Yeast Autolysate Supplement may be added. -For Chocolate Blood Agar, prepare single-strength medium using 3.6 grams in 100 ml of distilled water. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and add 5% v/v defibrited blood. -Mix well and heat at 80°C for 10 minutes.

-Dissolve the 36.0 grams in 500 ml purified / distilled water, to make a double strength base. -Heat to boiling to dissolve the medium completely. -Separately suspend 10 grams of Haemoglobin powder in 500 ml purified / distilled water (2% solution) to make a uniform solution. Separately sterilize both the solutions by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C. -Mix both the solutions uniformly and aseptically add the rehydrated contents of two vials of GC Supplement w/ Antibiotics. Mix well and pour into sterile Petri plates. -To increase the selectivity of medium antibiotic supplements such as V.C.N. Supplement or V.C.N.T. Supplement or Linco T Supplement or Vanclo T Supplement (two vials per litre) may be added. -To enhance the nutritiol properties of medium, Vitamins amino growth supplement or Yeast Autolysate Supplement may be added. -For Chocolate Blood Agar, prepare single-strength medium using 3.6 grams in 100 ml of distilled water. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and add 5% v/v defibrited blood. -Mix well and heat at 80°C for 10 minutes.