-Dissolve 54.92 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement and 2 vials of Fraser Supplement to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment.
-Mix well and dispense in tubes or flasks as desired.
-Dissolve 54.92 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement and 2 vials of Fraser Supplement to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment.
-Mix well and dispense in tubes or flasks as desired.
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