FLUID TETRATHIONATE MEDIUM (as per USP)
-Dissolve 46.0 grams in 970 ml distilled water and heat just to boiling. DO NOT AUTOCLAVE. -Cool to 45-50°C. On the day of use add 20 ml iodine solution (iodine - 6 grams and potassium iodide - 5 grams in 20 ml distilled water) and 10 ml of 0.1% brilliant green solution. -Mix well and dispense in 10 ml quantities. Do not heat after the addition of iodine solution. Use the medium immediately after addition of iodine.
-Dissolve 46.0 grams in 970 ml distilled water and heat just to boiling. DO NOT AUTOCLAVE. -Cool to 45-50°C. On the day of use add 20 ml iodine solution (iodine - 6 grams and potassium iodide - 5 grams in 20 ml distilled water) and 10 ml of 0.1% brilliant green solution. -Mix well and dispense in 10 ml quantities. Do not heat after the addition of iodine solution. Use the medium immediately after addition of iodine.
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