FLUID SELENITE CYSTINE MEDIUM (SELENITE CYSTINE MEDIUM) (DOUBLE PACK)
-Dissolve 4.0 grams of Part II in 1000 ml purified/ distilled water.
-Add 19.01 grams of Part I. Mix well. Warm to dissolve the medium completely.
-Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of tube / bottle).
Note: Instead of Part II, Sodium Biselenite discs (1 disc per 10 ml of the medium) or Sodium Biselenite Bud (1 bud per 100ml of medium) can be added to the medium after boiling.
-Dissolve 4.0 grams of Part II in 1000 ml purified/ distilled water.
-Add 19.01 grams of Part I. Mix well. Warm to dissolve the medium completely.
-Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of tube / bottle).
Note: Instead of Part II, Sodium Biselenite discs (1 disc per 10 ml of the medium) or Sodium Biselenite Bud (1 bud per 100ml of medium) can be added to the medium after boiling.
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