FLUCONAZOLE TESTING MEDIUM (TWIN PACK)
-Part I - Dissolve 2.0 grams of Part I in 100 ml purified / distilled water, add 0.1 ml phosphate buffer to adjust the pH to 7.5.
-Heat to boiling to dissolve the agar particles completely and then sterilize by autoclaving at 115°C for 10 minutes.
-Part II - Dissolve 29.31 grams of Part II in 900 ml purified / distilled water.
-Mix well, add 2 gram of sodium bicarbote, after stirring make up the total volume to 1 litre with distilled water. Sterilize by filtration. The medium can be kept at 4°C for two weeks.
-Complete medium is prepared by aseptically adding equal volume of molten Part I (previously cooled to 50°C) and Part II.
-Mix thoroughly and dispense.
-Part I - Dissolve 2.0 grams of Part I in 100 ml purified / distilled water, add 0.1 ml phosphate buffer to adjust the pH to 7.5.
-Heat to boiling to dissolve the agar particles completely and then sterilize by autoclaving at 115°C for 10 minutes.
-Part II - Dissolve 29.31 grams of Part II in 900 ml purified / distilled water.
-Mix well, add 2 gram of sodium bicarbote, after stirring make up the total volume to 1 litre with distilled water. Sterilize by filtration. The medium can be kept at 4°C for two weeks.
-Complete medium is prepared by aseptically adding equal volume of molten Part I (previously cooled to 50°C) and Part II.
-Mix thoroughly and dispense.
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