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Emb Agar, Levine
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MLSIGNUP100
Expiring in 1days 17hr11min47sec
EMB AGAR, LEVINE
-Dissolve the 37.5 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend
the precipitate which is an essential part of the medium.
-Dissolve the 37.5 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend
the precipitate which is an essential part of the medium.
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