EMB AGAR, LEVINE (as per USP)
-Dissolve 37.46 grams in 1000 ml purified / distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING. -Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium. -Mix well and pour into sterile Petri plates. Precaution: Store the medium away from light to avoid photo oxidation.
-Dissolve 37.46 grams in 1000 ml purified / distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING. -Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium. -Mix well and pour into sterile Petri plates. Precaution: Store the medium away from light to avoid photo oxidation.
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