-Dissolve 27.46 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely. Add desired carbohydrate or other test substance in desired concentration.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. DO NOT OVERHEAT.
-Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium.
-Mix well and pour into sterile Petri plates.
Precaution: Store the medium away from light to avoid photo oxidation.
-Dissolve 27.46 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely. Add desired carbohydrate or other test substance in desired concentration.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. DO NOT OVERHEAT.
-Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium.
-Mix well and pour into sterile Petri plates.
Precaution: Store the medium away from light to avoid photo oxidation.
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