-Dissolve 1 grams in 180 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 20ml of sterile Oleic Albumin Supplement and 5000 to 10000 units of Penicillin.
-Mix well and dispense in sterile tubes.
-Dissolve 1 grams in 180 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 20ml of sterile Oleic Albumin Supplement and 5000 to 10000 units of Penicillin.
-Mix well and dispense in sterile tubes.
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