DOUBLE MODIFIED LYSINE IRON AGAR BASE
-Dissolve 63.12 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE.
-Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Novobiocin supplement.
-Mix well and dispense into sterile Petri plates.
-Dissolve 63.12 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE.
-Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Novobiocin supplement.
-Mix well and dispense into sterile Petri plates.
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