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Differential Reinforced Clostridial Broth Base
INTENDED USE
For cultivation of Clostridia from water.
MLSIGNUP100
Expiring in 0days 5hr6min23sec
DIFFERENTIAL REINFORCED CLOSTRIDIAL BROTH BASE
PRODUCT SUMMARY AND EXPLANATION
Hirsch and Grinstead originally described Differential Reinforced Clostridial Medium to initiate the growth from small inoculum and get a higher Clostridial count. Later, Barnes and Ingram used the medium to develop vegetative cells in
assays of Clostridium perfringens. This medium is developed for the isolation of sulphite-reducing Clostridia from food and for their enumeration in water by multiple tube method. Differential Reinforced Clostridial Broth is used to determine the count of sulphite reducing bacteria by MPN technique.
PRINCIPLE
The medium consists of Peptone, Beef extract, yeast extract, starch, sodium acetate which provide essential nutrients for bacterial metabolism. Glucose is the fermentable carbohydrate and serves as carbon and energy source. L-cysteine hydrochloride acts as reducing agent. Sodium sulphite and ferric citrate are added as indicators. Sulphite reducing clostridia produce sulphide from sulphite, which results in the formation of black coloured medium.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light yellow coloured, clear solution without any precipitate.
pH (at 25°C) : 7.2 ± 0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Peptone | 10.000 |
| Beef extract | 10.000 |
| Yeast extract | 1.500 |
| Starch | 1.000 |
| Sodium acetate, hydrated | 5.000 |
| Dextrose (Glucose) | 1.000 |
| L-Cysteine hydrochloride | 0.500 |
INSTRUCTION FOR USE
- Dissolve 29 grams in 1000 ml purified/distilled water Heat to boiling to dissolve the medium completely Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
- Cool to 45-50°C. Just before use add 0.5 ml filter sterilized solution, prepared by mixing equal volumes of 4% w/v solution of sodium sulphite and 7% w/v ferric citrate, to 25 ml of single strength medium or 0.4 ml and 2 ml to 10 mland 50 ml of double strength medium respectively. Mix well.
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