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Differential Reinforced Clostridial Broth Base
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INTENDED USE
For cultivation of Clostridia from water.
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MLSIGNUP100
Expiring in 0days 13hr5min27sec
DIFFERENTIAL REINFORCED CLOSTRIDIAL BROTH BASE
-Dissolve 29 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C. Just before use add 0.5 ml filter sterilized solution, prepared by mixing equal volumes of 4% w/v solution of sodium sulphite and 7% w/v ferric citrate, to 25 ml of single strength medium or 0.4 ml and 2 ml to 10 ml and 50 ml of double strength medium respectively. Mix well.
-Dissolve 29 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C. Just before use add 0.5 ml filter sterilized solution, prepared by mixing equal volumes of 4% w/v solution of sodium sulphite and 7% w/v ferric citrate, to 25 ml of single strength medium or 0.4 ml and 2 ml to 10 ml and 50 ml of double strength medium respectively. Mix well.
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