Dichloran Rose Bengal Chloramphenicol Agar (Drbc Agar) (As Per Iso 21527)

PRODUCT SUMMARY AND EXPLANATION
DRBC Agar is a selective medium that supports good growth of yeasts and molds. This medium is formulated as described by King et. al and is recommended for selective isolation of fungi especially from food samples. DRBC Agar is a modification of Rose Bengal Chloramphenicol Agar which contains Dichloran as an additional component. The composition and performance criteria of this medium are as per the specifications laid down in ISO 21527-1:2008.

PRINCIPLE
Medium contains Peptic digest of animal tissue which serves as a source of nitrogen, vitamins and minerals. Dextrose is a carbon and energy source. Magnesium sulfate provides divalent cations and sulfate. Monopotassium phosphate is a buffering agent. Dichloran is an antifungal agent used to reduce the colony diameter of spreading fungi. The presence
of rose 1engal in the medium suppresses the growth of bacteria and restricts the size and colonies of the more rapidly growing molds. Chloramphenicol is included to inhibit the growth of bacteria present in environmental and food samples. Inhibition of growth of bacteria and restriction of spreading of more-rapidly growing molds aids in the isolation of slow-growing fungi by preventing their overgrowth by more-rapidly growing species. Additionally, Rose Bengal is taken by yeast and molds colonies, which allows these colonies to be easily recognized and enumerated.

QUALITY CONTROL SPECIFICATIONS
Appearance of Dehydrated powder : Light yellow to pink, Homogeneous free flowing powder
Appearance of Prepared medium : Pink coloured, clear to slightly opalescent gel
pH (at 25°C) : 5.6± 0.2