DIAGNOSTIC STUART’S UREA BROTH BASE (UREA BROTH BASE)
-Dissolve 18.71 grams in 950 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 55°C.
-Aseptically add 50 ml of sterile 40% Urea solution. Mix well and distribute in 10 ml amounts into sterile tubes.
-Dissolve 18.71 grams in 950 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 55°C.
-Aseptically add 50 ml of sterile 40% Urea solution. Mix well and distribute in 10 ml amounts into sterile tubes.
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