DIAGNOSTIC SENSITIVITY TEST AGAR (DST AGAR)
-Dissolve 43.04 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-For blood agar
-Dissolve 43.04 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-For blood agar, cool the base to 45-50°C and add 7% v/v sterile defibrited horse blood aseptically.
-Mix well with gentle rotation and pour into sterile Petri plates.
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