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Dextrose Agar Base, Emmons (Sabouraud Dextrose Agar Base, Modified)
INTENDED USE
For selective cultivation of pathogenic fungi.
MLSIGNUP100
Expiring in 13days 17hr6min21sec
DEXTROSE AGAR BASE, EMMONS (SABOURAUD DEXTROSE AGAR BASE, MODIFIED)
PRODUCT SUMMARY AND EXPLANATION
Sabouraud Dextrose Agar is Carliers modifications of the formulation described by Sabouraud for the cultivation of fungi, particularly those associated with skin infections. Sabouraud Dextrose Agar Base, Modified is the modification of
Sabouraud medium as described by Emmons. It has reduced dextrose content and a neutral pH. Though the low pH of this medium is favorable for the growth of fungi especially dermatophytes, some fungi are inhibited. Emmons modified
the original formulation by adjusting the pH close to neutral to increase the recovery of fungi and by reducing the dextrose content from 40 to 20 g/l.
PRINCIPLE
Peptone special is the source of nitrogenous growth factors. Dextrose provides as an energy source. The addition of antibiotics increases the selectivity of the medium.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light amber coloured clear to slightly opalescent gel forms in Petri plates.
pH (at 25°C) : 7.0±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Dextrose (Glucose) | 20.000 |
| Peptone, special | 10.000 |
| Agar | 17.000 |
INSTRUCTION FOR USE
- Dissolve 23.5 grams in 500 ml distilled water
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes
- Cool to 45-50°C and aseptically add the rehydrated contents of 1 vial of CC Supplement.
- Mix well before pouring in sterile Petri plates.
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