Free Shipping for all Order of ₹500
COD payment is available.
Nutrient Agar (Oxidase) (Veg.)
NUTRIENT AGAR (OXIDASE) (VEG.) for confirmation of oxidase production by microorganisms.
MLSIGNUP100
Expiring in 8days 11hr11min50sec
PRODUCT SUMMARY AND EXPLANATION
Nutrient Agar (Oxidase) (Veg) is prepared by completely replacing animal based peptones with veg peptones. This
medium is the modification of Nutrient Agar recommended by APHA for differentiation of the coliform bacteria on the basis of presence of enzyme cytochrome oxidase. Cytochrome oxidase is an iron-containing porphyrin enzyme that participates in the electron transfer mechanisms and in the nitrate metabolic pathways of some bacteria. Nutrient Veg Agar plates are streak inoculated to obtain isolated colonies. The isolated colony is used for oxidase testing on an impregnated filter paper. A dark purple colour that develops within 10 seconds is a positive oxidase test. Coliform bacteria are oxidase negative.
PRINCIPLE
The medium consists of Veg Peptone and veg extract no.1 that provide nitrogenous compounds, carbon, sulphur and
trace ingredients. Sodium chloride maintains osmotic equilibrium.
| COMPOSITION |
| Ingredients | Gms / Ltr |
| Veg Peptone | 1.000 |
| Veg extract no.1 | 1.000 |
| Agar | 15.000 |
| Sodium chloride | 5.000 |
INSTRUCTION FOR USE
- Dissolve 22.0 grams in 1000 ml purified/distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
- Mix well and pour into sterile Petri plates.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Yellow coloured clear to slightly opalescent gel forms in Petri plates.
pH (at 25°C) : 7.3 ± 0.2
The information below is required for social login
Sign In
Create New Account