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Emb Broth
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MLSIGNUP100
Expiring in 10days 4hr6min32sec
EMB BROTH
-Dissolve 22.46 grams in 1000 ml purified / distilled water.
-Mix until suspension is uniform. Heat if necessary to dissolve the medium completely.
-Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 psi pressure (121° C) for 15 minutes. AVOID OVERHEATING.
-Cool to 45-50°C. Shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculant precipitate.
-Dissolve 22.46 grams in 1000 ml purified / distilled water.
-Mix until suspension is uniform. Heat if necessary to dissolve the medium completely.
-Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 psi pressure (121° C) for 15 minutes. AVOID OVERHEATING.
-Cool to 45-50°C. Shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculant precipitate.
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