Dichloran Glycerol Medium Base W/Rose Bengal (Veg.)

PRODUCT SUMMARY AND EXPLANATION
Dichloran Medium Base with Rose Bengal (Veg) is prepared by replacing animal based peptone with vegetable peptones which is free from BSE/TSE. Dichloran Medium Base with Rose Bengal (Veg) is the modification of Dichloran Medium Base with Rose Bengal which is formulated as described by King et al, which is a modification of Rose Bengal Chloramphenicol Agar. It is recommended for the selective isolation and enumeration of yeasts and moulds of importance in food spoilage. Dichloran Medium Base with Rose Bengal (Veg) is the modification of this medium by replacing animal based peptone with vegetable peptones and it serves the same purpose.

PRINCIPLE
The medium consists of Veg Peptone which provides nitrogen, vitamins and minerals. Dextrose is a carbohydrate source. Phosphate buffers the medium. Magnesium sulfate provides divalent cations and sulfate. Dichloran is an antifungal agent, added to the medium to reduce colony diameters of spreading fungi. Rose bengal exhibits an improved inhibitory activity at pH 5.6 and therefore the final pH of the medium is reduced to 5.6 for the inhibition of spreading fungi. The presence of rose bengal in the medium suppresses the growth of bacteria and restricts the size and height of colonies of the more rapidly growing moulds. The concentration of rose bengal is reduced for optimal performance with dichloran. Additionally, rose bengal is taken up by yeast and mold colonies, which allows these colonies to be easily recognized and enumerated.