Buffered Charcoal Yeast Extract Agar Medium (Iso)

-Dissolve 35.00 grams in 1000ml distilled water. -Add 2.8 grams KOH pellets and mix to dissolve. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at (121 ± 3) °C for (15 ± 1) minutes. -Cool to 45-51°C. -Aseptically add sterile rehydrated contents of 2 vials each of Legionella Supplement (Part A and Part B) (TS 019). -Mix well and pour with constant stirring to ensure that charcoal particles get evenly distributed. For additiol selectivity: -For BCYE+AB Agar: Aseptically add the rehydrated contents of one vial of PCP Supplement (TS 254) -For GVPC Agar: Aseptically add rehydrated contents of 2 vials of Legionella (GVPC) Selective Supplement (TS 115)." -For Modified Wadowsky Yee (MWY) Agar: Aseptically add the rehydrated contents of one vial of Legionella Selective Supplement IV (MWY) (TS 018). Note: Medium w/o L-Cysteine may be prepared by aseptically adding contents of two vials of Legionella Supplement (TS 019, Part B only).

-Dissolve 35.00 grams in 1000ml distilled water. -Add 2.8 grams KOH pellets and mix to dissolve. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at (121 ± 3) °C for (15 ± 1) minutes. -Cool to 45-51°C. -Aseptically add sterile rehydrated contents of 2 vials each of Legionella Supplement (Part A and Part B) (TS 019). -Mix well and pour with constant stirring to ensure that charcoal particles get evenly distributed. For additiol selectivity: -For BCYE+AB Agar: Aseptically add the rehydrated contents of one vial of PCP Supplement (TS 254) -For GVPC Agar: Aseptically add rehydrated contents of 2 vials of Legionella (GVPC) Selective Supplement (TS 115)." -For Modified Wadowsky Yee (MWY) Agar: Aseptically add the rehydrated contents of one vial of Legionella Selective Supplement IV (MWY) (TS 018). Note: Medium w/o L-Cysteine may be prepared by aseptically adding contents of two vials of Legionella Supplement (TS 019, Part B only).