BUFFERED CHARCOAL YEAST EXTRACT AGAR MEDIUM (ISO)
-Dissolve 35.00 grams in 1000ml distilled water.
-Add 2.8 grams KOH pellets and mix to dissolve.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at (121 ± 3) °C for (15 ± 1) minutes.
-Cool to 45-51°C.
-Aseptically add sterile rehydrated contents of 2 vials each of Legionella Supplement (Part A and Part B) (TS 019).
-Mix well and pour with constant stirring to ensure that charcoal particles get evenly distributed. For additiol selectivity:
-For BCYE+AB Agar: Aseptically add the rehydrated contents of one vial of PCP Supplement (TS 254)
-For GVPC Agar: Aseptically add rehydrated contents of 2 vials of Legionella (GVPC) Selective Supplement (TS 115)."
-For Modified Wadowsky Yee (MWY) Agar: Aseptically add the rehydrated contents of one vial of Legionella Selective Supplement IV (MWY) (TS 018).
Note: Medium w/o L-Cysteine may be prepared by aseptically adding contents of two vials of Legionella Supplement (TS 019, Part B only).
-Dissolve 35.00 grams in 1000ml distilled water.
-Add 2.8 grams KOH pellets and mix to dissolve.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at (121 ± 3) °C for (15 ± 1) minutes.
-Cool to 45-51°C.
-Aseptically add sterile rehydrated contents of 2 vials each of Legionella Supplement (Part A and Part B) (TS 019).
-Mix well and pour with constant stirring to ensure that charcoal particles get evenly distributed. For additiol selectivity:
-For BCYE+AB Agar: Aseptically add the rehydrated contents of one vial of PCP Supplement (TS 254)
-For GVPC Agar: Aseptically add rehydrated contents of 2 vials of Legionella (GVPC) Selective Supplement (TS 115)."
-For Modified Wadowsky Yee (MWY) Agar: Aseptically add the rehydrated contents of one vial of Legionella Selective Supplement IV (MWY) (TS 018).
Note: Medium w/o L-Cysteine may be prepared by aseptically adding contents of two vials of Legionella Supplement (TS 019, Part B only).
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