TERGITOL - 7 AGAR BASE (as per BIS)
1. Dissolve 33.12 grams in 1000 ml distilled water.
2. Gently heat to boiling with swirling to dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
4. Cool to 45-50°C.
5. Aseptically add 3 ml of Triphenyl Tetrazolium Chloride (TTC) Solution (TS 042), if desired.
6. Mix well and pour into sterile petri plates.
1. Dissolve 33.12 grams in 1000 ml distilled water.
2. Gently heat to boiling with swirling to dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
4. Cool to 45-50°C.
5. Aseptically add 3 ml of Triphenyl Tetrazolium Chloride (TTC) Solution (TS 042), if desired.
6. Mix well and pour into sterile petri plates.
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