HUGH LEIFSON MEDIUM (as per BIS)
1. Dissolve 20.33 grams in 1000ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Dispense in test tubes in duplicate for aerobic and aerobic fermentation.
4. Sterilize by autoclaving at 10 psi (115°C) for 20 minutes.
5. Cool the tubed medium in upright position.
1. Dissolve 20.33 grams in 1000ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Dispense in test tubes in duplicate for aerobic and aerobic fermentation.
4. Sterilize by autoclaving at 10 psi (115°C) for 20 minutes.
5. Cool the tubed medium in upright position.
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