BUFFERED PEPTONE WATER (as per BIS)
1. Dissolve 20.00 grams in 1000ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Distribute 50 ml amount into each flask.
4. Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
5. Cool at room temperature prior to use.
1. Dissolve 20.00 grams in 1000ml distilled water.
2. Gently heat to boiling with gentle swirling and dissolve the medium completely.
3. Distribute 50 ml amount into each flask.
4. Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
5. Cool at room temperature prior to use.
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