1. Dissolve 40 grams in 1000 ml distilled water.
2. Gently heat to boiling with swirling to dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
4. Cool to 45-50°C and dispense as desired.
1. Dissolve 40 grams in 1000 ml distilled water.
2. Gently heat to boiling with swirling to dissolve the medium completely.
3. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
4. Cool to 45-50°C and dispense as desired.
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