-Dissolve 28.51 grams in 1000 ml purified / distilled water. -Heat to boiling to dissolve the medium completely. -Dispense in tubes in 8-10 ml amounts. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C and add appropriate carbohydrate (0.5 to 1.0% if desired). -Mix well and allow the tubed medium to cool in an upright position.
-Dissolve 28.51 grams in 1000 ml purified / distilled water. -Heat to boiling to dissolve the medium completely. -Dispense in tubes in 8-10 ml amounts. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C and add appropriate carbohydrate (0.5 to 1.0% if desired). -Mix well and allow the tubed medium to cool in an upright position.
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