Columbia Blood Agar Base

-Dissolve 44.0 grams of in 1000 ml purified/ distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C before adding heat sensitive compounds. -For Blood Agar: Add 5% v/v sterile defibrited sheep blood to sterile cool base. -For Chocolate Agar: Add 10% v/v sterile defibrited sheep blood to sterile cool base. Heat to 80°C for 10 minutes with constant agitation. The medium can be made selective by adding different antimicrobials to sterile base. -For Brucella species: Add rehydrated contents of 1 vial of Brucella Selective Supplement to 500 ml sterile molten base. -For Campylobacter species: Add rehydrated contents of 1 vial of Campylobacter Supplement- I (Blaser-Wang) or Campylobacter Supplement- II, (Butzler) or Campylobacter Supplement- III (Skirrow) or Campylobacter Selective Supplement or Campylobacter Supplement- VI (Butzler) to 500 ml sterile molten base along with rehydrated contents of 1 vial of Campylobacter Growth Supplement and 5-7% v/v horse or sheep blood. -For Gardnerella species: Add rehydrated contents of 1 vial of G.Vagilis Selective Supplement to 500 ml sterile molten base. -For Cocci: Add rehydrated contents of 1 vial of Staph-Strepto Supplement or Strepto Supplement or Streptococcus Selective Supplement to 500 ml sterile molten base

-Dissolve 44.0 grams of in 1000 ml purified/ distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool to 45-50°C before adding heat sensitive compounds. -For Blood Agar: Add 5% v/v sterile defibrited sheep blood to sterile cool base. -For Chocolate Agar: Add 10% v/v sterile defibrited sheep blood to sterile cool base. Heat to 80°C for 10 minutes with constant agitation. The medium can be made selective by adding different antimicrobials to sterile base. -For Brucella species: Add rehydrated contents of 1 vial of Brucella Selective Supplement to 500 ml sterile molten base. -For Campylobacter species: Add rehydrated contents of 1 vial of Campylobacter Supplement- I (Blaser-Wang) or Campylobacter Supplement- II, (Butzler) or Campylobacter Supplement- III (Skirrow) or Campylobacter Selective Supplement or Campylobacter Supplement- VI (Butzler) to 500 ml sterile molten base along with rehydrated contents of 1 vial of Campylobacter Growth Supplement and 5-7% v/v horse or sheep blood. -For Gardnerella species: Add rehydrated contents of 1 vial of G.Vagilis Selective Supplement to 500 ml sterile molten base. -For Cocci: Add rehydrated contents of 1 vial of Staph-Strepto Supplement or Strepto Supplement or Streptococcus Selective Supplement to 500 ml sterile molten base