CFC AGAR BASE (CEPHALOTHIN-SODIUM FUSIDATE-CETRIMIDE AGAR)
-Dissolve 52.4 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add sterile rehydrated contents of two vials of Modified CFC Selective Supplement.
-Mix well and pour into sterile Petri plates.
Note: Do not keep the molten agar for longer than 4 hours.
-Dissolve 52.4 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add sterile rehydrated contents of two vials of Modified CFC Selective Supplement.
-Mix well and pour into sterile Petri plates.
Note: Do not keep the molten agar for longer than 4 hours.
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