CETRIMIDE AGAR BASE (W 1.3% AGAR)
-Dissolve 44.7 grams in 1000 ml distilled water containing 10 ml glycerin/glycerol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-If desired, rehydrated contents of 1 vial of lidixic Selective Supplement may be added aseptically to 1000 ml medium previously cooled to 45- 50°C.
-Mix well and pour into sterile Petri plates.
-Dissolve 44.7 grams in 1000 ml distilled water containing 10 ml glycerin/glycerol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-If desired, rehydrated contents of 1 vial of lidixic Selective Supplement may be added aseptically to 1000 ml medium previously cooled to 45- 50°C.
-Mix well and pour into sterile Petri plates.
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