CARRY-BLAIR MEDIUM BASE (TRANSPORT MEDIUM W/O CHARCOAL)
-Suspend 12.6 grams in 991 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Cool to 45-50°C and aseptically add 9 ml of 1% aqueous calcium chloride solution.
-Adjust pH to 8.4 if necessary. Distribute in 7 ml amounts in screw-capped tubes.
-Steam for 15 minutes and cool and tighten the caps.
-Suspend 12.6 grams in 991 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Cool to 45-50°C and aseptically add 9 ml of 1% aqueous calcium chloride solution.
-Adjust pH to 8.4 if necessary. Distribute in 7 ml amounts in screw-capped tubes.
-Steam for 15 minutes and cool and tighten the caps.
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