Brucella Selective Supplement

Rehydrate the contents of 1 vial aseptically with 10 ml of 50% methanol. Shake to form a uniform suspension. Add the contents to 500 ml of sterile, molten, cooled (45-50°C) media such as, TM 041- BLOOD AGAR BASE No. 2 / TM 1842 - BLOOD AGAR BASE NO. 2 (ISO 11290-1:2017) / TM 2011 - BLOOD AGAR BASE NO. 2 WITH 1.2% AGAR / TM 071 - COLUMBIA BLOOD AGAR BASE / TMV 071 - COLUMBIA BLOOD AGAR BASE (VEG.) / TM 2044 - COLUMBIA BLOOD AGAR BASE W/ 1% AGAR with 5-10% v/v ictivated horse serum and 1% w/v sterile dextrose / TMV 514 - BRUCELLA AGAR BASE (VEG.) / TM 680 - BRUCELLA AGAR BASE, MODIFIED / TM 050 - BRUCELLA BROTH BASE / TM 682 - BRUCELLA SELECTIVE MEDIUM BASE / TM 1513 - BRUCELLA AGAR BASE W/ 1.0% DEXTROSE with 5-10% v/v ictivated horse serum. Mix well and pour into sterile petri plates / tubes.

Rehydrate the contents of 1 vial aseptically with 10 ml of 50% methanol. Shake to form a uniform suspension. Add the contents to 500 ml of sterile, molten, cooled (45-50°C) media such as, TM 041- BLOOD AGAR BASE No. 2 / TM 1842 - BLOOD AGAR BASE NO. 2 (ISO 11290-1:2017) / TM 2011 - BLOOD AGAR BASE NO. 2 WITH 1.2% AGAR / TM 071 - COLUMBIA BLOOD AGAR BASE / TMV 071 - COLUMBIA BLOOD AGAR BASE (VEG.) / TM 2044 - COLUMBIA BLOOD AGAR BASE W/ 1% AGAR with 5-10% v/v ictivated horse serum and 1% w/v sterile dextrose / TMV 514 - BRUCELLA AGAR BASE (VEG.) / TM 680 - BRUCELLA AGAR BASE, MODIFIED / TM 050 - BRUCELLA BROTH BASE / TM 682 - BRUCELLA SELECTIVE MEDIUM BASE / TM 1513 - BRUCELLA AGAR BASE W/ 1.0% DEXTROSE with 5-10% v/v ictivated horse serum. Mix well and pour into sterile petri plates / tubes.