Brucella Agar Base

-Dissolve 21.55 grams in 500 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C. -Mix well and pour into sterile Petri plates. If required, for additiol selectivity of Brucella species: -Aseptically add sterile 5% v/v ictivated Horse Serum (ictivated by heating at 56°C for 30 minutes) and rehydrated contents of one vial of Brucella Selective Supplement. -For Campylobacter: Add rehydrated contents of 1 vial of Campylobacter Supplement-I (Blaser-Wang) or Campylobacter Supplement-II (Butzler) or Campylobacter Supplement-III (Skirrow) and 5-7% defibrited sheep blood to 500 ml sterile medium. For growth enhancement add rehydrated contents of 1 vial of Campylobacter Growth Supplement. Mix well before pouring into sterile Petri plates.

-Dissolve 21.55 grams in 500 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C. -Mix well and pour into sterile Petri plates. If required, for additiol selectivity of Brucella species: -Aseptically add sterile 5% v/v ictivated Horse Serum (ictivated by heating at 56°C for 30 minutes) and rehydrated contents of one vial of Brucella Selective Supplement. -For Campylobacter: Add rehydrated contents of 1 vial of Campylobacter Supplement-I (Blaser-Wang) or Campylobacter Supplement-II (Butzler) or Campylobacter Supplement-III (Skirrow) and 5-7% defibrited sheep blood to 500 ml sterile medium. For growth enhancement add rehydrated contents of 1 vial of Campylobacter Growth Supplement. Mix well before pouring into sterile Petri plates.