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Brewer Thioglycollate Medium
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MLSIGNUP100
Expiring in 1days 8hr6min47sec
BREWER THIOGLYCOLLATE MEDIUM
-Dissolve 40.5 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Note: If more than the upper one third layer acquires bluish-green colour (absorbs oxygen), the dissolved oxygen can be removed by heating the medium in free flowing steam for 5-10 minutes or in a water bath untill the green colour disappears, and the prepared medium should be stored in the dark till use.
-Dissolve 40.5 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Note: If more than the upper one third layer acquires bluish-green colour (absorbs oxygen), the dissolved oxygen can be removed by heating the medium in free flowing steam for 5-10 minutes or in a water bath untill the green colour disappears, and the prepared medium should be stored in the dark till use.
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