BREWER THIOGLYCOLLATE MEDIUM, MODIFIED
-Dissolve 20.6 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or flasks as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Note: If more than the upper one third medium has acquired a green colour, the medium may be restored once by heating in a water bath or free flowing steam until the green colour disappears.
-Dissolve 20.6 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or flasks as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Note: If more than the upper one third medium has acquired a green colour, the medium may be restored once by heating in a water bath or free flowing steam until the green colour disappears.
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