-Dissolve 52.0 grams in 1000 ml purified / distilled water.
-Gently heat to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
-Cool to 45-50°C. If desired add 20 unit of Penicillin and 40μg Streptomycin/ml.
-Dissolve 52.0 grams in 1000 ml purified / distilled water.
-Gently heat to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121°C) for 15 minutes.
-Cool to 45-50°C. If desired add 20 unit of Penicillin and 40μg Streptomycin/ml.
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