Blood Agar Base No 2

-Dissolve 21.25 grams in 500 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45 - 50°C and aseptically add 7% v/v sterile defibrited blood. -For Brucella species: Add rehydrated contents of 1 vial of Brucella Selective Supplement to 500 ml sterile molten base. -For Campylobacter species: Add rehydrated contents of 1 vial of Campylobacter Supplement - I or Campylobacter Supplement - II or Campylobacter Supplement - III or Campylobacter Growth Supplement to 500 ml sterile molten base. -For Streptococcus species: Add rehydrated contents of 1 vial of Strepto Supplement to 500 ml sterile molten base. Mix well and pour into sterile Petri plates.

-Dissolve 21.25 grams in 500 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45 - 50°C and aseptically add 7% v/v sterile defibrited blood. -For Brucella species: Add rehydrated contents of 1 vial of Brucella Selective Supplement to 500 ml sterile molten base. -For Campylobacter species: Add rehydrated contents of 1 vial of Campylobacter Supplement - I or Campylobacter Supplement - II or Campylobacter Supplement - III or Campylobacter Growth Supplement to 500 ml sterile molten base. -For Streptococcus species: Add rehydrated contents of 1 vial of Strepto Supplement to 500 ml sterile molten base. Mix well and pour into sterile Petri plates.