BISMUTH SULPHITE AGAR MEDIUM (as per USP)
-Dissolve 52.32 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely, do not overheat or sterilize in autoclave or by fractiol sterilization since overheating may destroy the selectivity of the medium.
-Transfer to a water bath maintained at about 50°C.
-The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the fil gel, which should be dispersed before pouring into the sterile Petri plates.
-Dissolve 52.32 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely, do not overheat or sterilize in autoclave or by fractiol sterilization since overheating may destroy the selectivity of the medium.
-Transfer to a water bath maintained at about 50°C.
-The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the fil gel, which should be dispersed before pouring into the sterile Petri plates.
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