BISMUTH SULPHITE AGAR (as per IP) (DOUBLE PACK) (PART - I )
-Dissolve 40.4 grams of Part I (Solution 1) in 1000 ml ditilled water.
-Heat to boiling to dissolve the medium completely. Sterilize by maintaining at 115ºC for 30 minutes.
-Suspend 22.54 grams the equivalent weight of dehydrated medium per litre) of Part II (Solution 1) in 100 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-DO NOT AUTOCLAVE Add one volume of Part II solution to ten volumes of Part I solution previously melted and cooled at a temperature of 55°C.
-The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the fil gel, which should be dispersed before pouring into the sterile Petri plates.
-Dissolve 40.4 grams of Part I (Solution 1) in 1000 ml ditilled water.
-Heat to boiling to dissolve the medium completely. Sterilize by maintaining at 115ºC for 30 minutes.
-Suspend 22.54 grams the equivalent weight of dehydrated medium per litre) of Part II (Solution 1) in 100 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-DO NOT AUTOCLAVE Add one volume of Part II solution to ten volumes of Part I solution previously melted and cooled at a temperature of 55°C.
-The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the fil gel, which should be dispersed before pouring into the sterile Petri plates.
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