Tryptone - D

Tryptone-D is a pancreatic digest of casein and as such composed of a mixture of amino acids, including essential amino acids, and larger peptides. Casein is the main protein of milk and is a rich source of amino acid nitrogen. Amongst all amino acids especially Tryptophan is present in high concentrations. Due to the rich nutritional properties, Tryptone is added to media as an accelerator to increase the yield of organisms and is recommended where a rapid and luxuriant growth of microorganisms is required. It is one of the components of the commonly used LB Broth for the growth of recombinant Escherichia coli. It is widely used as a supplement for microbiological culture media, as the hydrolysate is a good nutrient.