-Dissolve 64.5 grams in 1000ml distilled water. -Gently heat to boiling with gentle swirling to dissolve the medium completely. -Dispense into tubes or flasks. -Sterilize by autoclaving at 15 psi (121oC) for 15 minutes. -Allow the tubed medium to solidify in a slanted position with a butt of 2.5cm deep or pour into sterile Petri plates.
-Dissolve 64.5 grams in 1000ml distilled water. -Gently heat to boiling with gentle swirling to dissolve the medium completely. -Dispense into tubes or flasks. -Sterilize by autoclaving at 15 psi (121oC) for 15 minutes. -Allow the tubed medium to solidify in a slanted position with a butt of 2.5cm deep or pour into sterile Petri plates.
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