AZOSPIRILLUM MEDIUM W/ 0.17% AGAR (DOUBLE PACK) (PART - I)
-Dissolve 8.08 grams of dehydrated Part I in 950 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 4 grams of Potassium hydroxide (Part II) dissolved in 50 ml of sterile distilled water to obtain pH of 6.8±0.2
-Dissolve 8.08 grams of dehydrated Part I in 950 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 4 grams of Potassium hydroxide (Part II) dissolved in 50 ml of sterile distilled water to obtain pH of 6.8±0.2
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