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Azospirillum Medium W/ 0.17% Agar (Double Pack) (Part - I)
INTENDED USE
For cultivation of Azospirillum species.
MLSIGNUP100
Expiring in 23days 17hr7min1sec
TM 638 – AZOSPIRILLUM MEDIUM W/ 0.17% AGAR (DOUBLE PACK)
PRODUCT SUMMARY AND EXPLANATION
Azospirillum species occur as free-living in soil or in association with the roots of cereal crops, grasses and tuber plants. Azospirillum species are plant-associated diazotrophs of the alpha subclass of Proteobacteria. Azospirillum Medium with
0.17% Agar is used for cultivation of Azospirillum species.
PRINCIPLE
Malic acid is used as the carbon source. Azospirillum species grow well in presence of Malic acid and are not overgrown by other nitrogen fixers. Dipotassium phosphate provides buffering effect and other inorganic salt ingredients provide necessary growth nutrients. Agar at 0.17% concentrations provides microaerophillic conditions necessary for nitrogen fixation by Azospirillum species.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Part I: Cream to yellow homogeneous free flowing powder Part II :White to cream pellets
Appearance of prepared medium : Light yellow to pale green coloured clear to slightly opalescent solution.
pH (at 25°C) : 6.8±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Malic acid | 5.000 |
| Dipotassium hydrogen phosphate | 0.500 |
| Ferrous sulphate | 0.500 |
| Manganese sulphate | 0.010 |
| Magnesium sulphate | 0.200 |
| Sodium chloride | 0.100 |
| Bromo thymol blue | 0.002 |
| Sodium molybdate | 0.002 |
| Calcium chloride | 0.020 |
| Agar | 1.750 |
| Potassium hydroxide | 4.000 |
INSTRUCTION FOR USE
- Dissolve 8.08 grams of dehydrated Part I in 950 ml purified / distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
- Cool to 45-50°C and aseptically add 4 grams of Potassium hydroxide (Part II) dissolved in 50 ml of sterile distilled water to obtain pH of 6.8±0.2
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