-Dissolve 14.5 grams (for single strength medium) or 23.2 grams (for concentrated medium) in 1000 ml distilled water containing 25 ml or 40 ml ethanol respectively.
-Heat to boiling to dissolve the medium completely. Distribute as desired in screw-capped bottles. Close the caps so that the seal in the lid just touches the lip of the bottle.
-Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
-Tighten the caps of the bottles immediately after removal from the autoclave to prevent loss of ethanol by evaporation.
-Dissolve 14.5 grams (for single strength medium) or 23.2 grams (for concentrated medium) in 1000 ml distilled water containing 25 ml or 40 ml ethanol respectively.
-Heat to boiling to dissolve the medium completely. Distribute as desired in screw-capped bottles. Close the caps so that the seal in the lid just touches the lip of the bottle.
-Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
-Tighten the caps of the bottles immediately after removal from the autoclave to prevent loss of ethanol by evaporation.
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