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Ashby’S Mannitol Agar
INTENDED USE
For isolation of Azotobacter species from soil.
MLSIGNUP100
Expiring in 23days 17hr7min15sec
TM 663 – ASHBY’S MANNITOL AGAR
PRODUCT SUMMARY AND EXPLANATION
Azotobacter is a genus of free-living diazotrophic bacteria which have the highest metabolic rate compared to any other microorganisms. Azotobacters are chemoorganotrophic, using sugars, alcohols and salts of organic acids for growth. Azotobacters can non-symbiotically fix atmospheric nitrogen aerobically due to their unique mode of metabolism. Besides the ability to fix atmospheric nitrogen, Azotobacter also synthesize biologically active substances, which
attributes to improving seed germination, plant growth etc. Ashby’s Mannitol Agar are formulated as described by Subba Rao. It is used for isolation of Azotobacter, a non-symbiotic nitrogen fixing bacteria which uses mannitol as a carbon source and atmospheric nitrogen as nitrogen source.
PRINCIPLE
Dipotassium phosphate provides buffering to the medium. Various essential ions required for promoting growth of Azotobacter are also available in this medium.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : White to cream homogeneous free flowing powder.
Appearance of prepared medium : Whitish opalescent gel forms in Petri plates.
pH (at 25°C) : 7.4±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Mannitol | 20.000 |
| Dipotassium hydrogen phosphate | 0.200 |
| Magnesium sulphate | 0.200 |
| Sodium chloride | 0.200 |
| Potassium sulphate | 0.100 |
| Calcium carbonate | 5.000 |
| Agar | 15.000 |
INSTRUCTION FOR USE
- Dissolve 40.7 grams in 1000 ml purified / distilled water.
- Heat just to boiling. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
- Mix well and pour into sterile Petri plates.
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