Cefaclor

Prepare sterile media plates of MUELLER HINTON AGAR (TM 339/TM 236) for rapidly growing aerobic organisms as per user’s instruction. The agar depth should be 4.0 ± 0.5 mm. Inoculate the plates using inoculum of turbidity comparable to that of standard 0.5 McFarland by lawn technique using a sterile cotton swab. Altertively, the inoculum can be standardized by other appropriate optical method (0.08 - 0.13 OD turbid suspension at 625 nm). Allow the inoculum to dry for 5 - 15 minutes with lid in place. Apply the antibiotic disc(s) aseptically, using sterile applicator or forceps. Place the antimicrobial discs with centers at least 24 mm apart. For fastidious organisms and for Penicillin and Cephalosporins, the discs should preferably be placed with centers 30 mm apart. Incubate immediately at 35 ±2°C and examine after 16-20 hours or longer, if necessary. For fastidious organisms incubate at appropriate temperature and time. Measure the zone of inhibition and record the diameters of the zones to the nearest millimeter using a calibrated instrument.

Prepare sterile media plates of MUELLER HINTON AGAR (TM 339/TM 236) for rapidly growing aerobic organisms as per user’s instruction. The agar depth should be 4.0 ± 0.5 mm. Inoculate the plates using inoculum of turbidity comparable to that of standard 0.5 McFarland by lawn technique using a sterile cotton swab. Altertively, the inoculum can be standardized by other appropriate optical method (0.08 - 0.13 OD turbid suspension at 625 nm). Allow the inoculum to dry for 5 - 15 minutes with lid in place. Apply the antibiotic disc(s) aseptically, using sterile applicator or forceps. Place the antimicrobial discs with centers at least 24 mm apart. For fastidious organisms and for Penicillin and Cephalosporins, the discs should preferably be placed with centers 30 mm apart. Incubate immediately at 35 ±2°C and examine after 16-20 hours or longer, if necessary. For fastidious organisms incubate at appropriate temperature and time. Measure the zone of inhibition and record the diameters of the zones to the nearest millimeter using a calibrated instrument.