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Antibiotic Assay Medium No 8 (Base Agar W/ Low Ph)
INTENDED USE
For microbiological assay of Mitomycin, Plicamycin and Vancomycin.
MLSIGNUP100
Expiring in 29days 2hr10min25sec
ANTIBIOTIC ASSAY MEDIUM No 8 (BASE AGAR W/ LOW pH)
PRODUCT SUMMARY AND EXPLANATION
Antibiotic Assay Media are used in the performance of antibiotic assays. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays. Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay. These media are prepared according to the specifications detailed in the USP and FDA.
PRINCIPLE
The medium contains beef extract, yeast extract and Peptone serves as a source of nutrients and growth factors.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light amber coloured opalescent gel forms in Petri plates.
pH (at 25°C) : 5.9±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Peptone | 6.000 |
| Yeast extract | 3.000 |
| Beef extract | 1.500 |
| Agar | 15.000 |
INSTRUCTION FOR USE
- Dissolve 25.5 grams in 1000 ml purified / distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
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