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Antibiotic Assay Medium No 32
INTENDED USE
For microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum of Bacillus subtilis ATCC 6633.
MLSIGNUP100
Expiring in 23days 17hr7min50sec
ANTIBIOTIC ASSAY MEDIUM No 32
PRODUCT SUMMARY AND EXPLANATION
This medium is formulated in accordance to FDA and is a modification of Antibiotic assay Medium No.1. This medium is used to develop incoulum of Bacillus subtilis for antibiotic assay.
PRINCIPLE
Essential nutrients, vitamins, mineral, trace elements and growth factors are supplied by Peptone, Tryptone, yeast extract and beef extract. Dextrose in the medium serves as the carbon source for stimulating the growth of the test
microorganism. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis, which is generally used as test organisms for plate assay of Dihydrostreptomycin and Vancomycin.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow coloured homogeneous free flowing powder. :
Appearance of prepared medium : Yellow coloured clear to slightly opalescent gel forms in Petri plates. :
pH (at 25°C) : 6.6±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Peptone | 6.000 |
| Tryptone | 4.000 |
| Yeast extract | 3.000 |
| Beef extract | 1.500 |
| Dextrose | 1.000 |
| Manganese sulphate | 0.300 |
| Agar | 15.000 |
INSTRUCTION FOR USE
- Suspend 30.8 gms in 1000ml purified/distilled water
- Heat to boiling to disslove the medium completely.
- Dispense and sterilise by autoclaving at 15 psi pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Dihydrostreptomycin and Vancomycin.
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