ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per USP)
-Dissolve 29.0 grams in 1000 ml purified / distilled water.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense into sterile tubes or flasks as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
Note: It is preferable to use freshly prepared medium, altertively it should be boiled and cooled just once prior to use as on reheating, toxic oxygen radicles are formed.
-Dissolve 29.0 grams in 1000 ml purified / distilled water.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense into sterile tubes or flasks as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
Note: It is preferable to use freshly prepared medium, altertively it should be boiled and cooled just once prior to use as on reheating, toxic oxygen radicles are formed.
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