AGAR MEDIUM M (TRIPLE SUGAR, IRON AGAR) (as per BP/EP)
-Disssolve 64.03 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified /distilled water.
-Heat to boiling to dissolve the medium completely.
-Mix well and distribute into test tubes.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes or as per validated cycle.
-Allow the medium to set in sloped form with a butt about 1 inch long.
-Disssolve 64.03 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified /distilled water.
-Heat to boiling to dissolve the medium completely.
-Mix well and distribute into test tubes.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes or as per validated cycle.
-Allow the medium to set in sloped form with a butt about 1 inch long.
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