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Agar Agar Type-I
INTENDED USE
Used as a solidifying agent in bacteriological routine laboratory work like preparation of microbiological culture media, plant tissue culture media, and for pharmaceutical preparations etc.
MLSIGNUP100
Expiring in 13days 12hr6min7sec
242 M - AGAR AGAR TYPE-I (For General Purpose, Bacteriological Grade)
PRODUCT SUMMARY AND EXPLANATION
Agar is a phycocolloid, water soluble polysaccharide, extracted from a group of red-purple marine algae (Class Rhodophyceae) including Gelidium and Gracilaria. Agar is normally used in final concentration of 1-2% for solidification
of culture media. Agar are routinely used as a solidifying agent because of several facts like, good transparency to allow identification of colony type, consistent gelling (32°C-40°C) and melting (approximately 85°C) temperatures, free from toxic substances that inhibits bacterial growth etc.
PRINCIPLE
Agar Agar Type I is used in routine bacteriological laboratory works like in preparation of bacterial, fungal, and plant tissue culture media, in pharmaceutical preparations, where clarity, compatibility are not of prime importance. When suspended in cold water, it swells but does not dissolve. However, it readily dissolves in boiling water and solubility is facilitated by soaking the powder in cold water. The main advantage of this agar is the absence of inhibitors, which could interfere in the micro-organisms growth. It has excellent transparency, high hysteresis and very reliable reproducibility.
QUALITY CONTROL SPECIFICATIONS
Appearance : Yellowish to Creamish colour free flowing powder.
Solubility (2% soln. at 90ºC) : Soluble in hot water. Near to clear.
Clarity (1% Soln. at 121ºC) : Near to clear, light yellowish colour jelly.
pH (2% Soln. at 25ºC) : 6.0 – 7.0
COMPOSITION
| Loss on drying (at 105ºC) | NMT – 18.0% |
| Acid Insoluble Ash | NMT – 0.5% |
| Gelling Temp. | 42 – 44ºC |
| Melting Temp. | 85 – 88ºC |
| Total Ash | NMT – 5.0% |
| Gel Strength (1.5% Gel) | NLT – 900 g/cm2 |
| Starch Test/ Gelatin Test | Negative |
| Microbial Test | Passes Test |
INSTRUCTION FOR USE
Agar Agar Type-I is a gelling agent used in the preparation of culture media and other bacteriological applications. It is generally used in a concentration of 1 - 2% for microbiological culture media preparations, 0.5% for motility testing and
0.1% maximum for anaerobes and microaerophiles growth.
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