-Suspend 31.5 grams in 1000 ml purified/distilled water. -Heat if necessary to dissolve the medium completely. -Distribute 10 ml amounts into tubes containing inverted Durham's tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 10 minutes
-Suspend 31.5 grams in 1000 ml purified/distilled water. -Heat if necessary to dissolve the medium completely. -Distribute 10 ml amounts into tubes containing inverted Durham's tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 10 minutes
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