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Park & Sander Enrichment Broth Base (Iso/Dis 10272:1995)
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MLSIGNUP100
Expiring in 2days 8hr6min32sec
PARK & SANDER ENRICHMENT BROTH BASE (ISO/DIS 10272:1995)
-Dissolve 29.35 grams in 940 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45°C and aseptically add 50 ml of sterile defibrited lysed horse blood and reconstituted contents of 1 vial of Park and Sanders Selective Supplement A. Mix well.
-Inoculate with food samples and incubate at 31 to 32°C (to recover injured cells) for 4 hours.
-Aseptically add reconstituted contents of 1 vial of Park and Sanders Selective Supplement B and incubate at 37°C for 2 hours, then at 42°C under a microaerobic atmosphere for additiol 40 to 42 hours with agitation at 100 rpm.
-Dissolve 29.35 grams in 940 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45°C and aseptically add 50 ml of sterile defibrited lysed horse blood and reconstituted contents of 1 vial of Park and Sanders Selective Supplement A. Mix well.
-Inoculate with food samples and incubate at 31 to 32°C (to recover injured cells) for 4 hours.
-Aseptically add reconstituted contents of 1 vial of Park and Sanders Selective Supplement B and incubate at 37°C for 2 hours, then at 42°C under a microaerobic atmosphere for additiol 40 to 42 hours with agitation at 100 rpm.
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